Immunohistochemical neuroinflammatory markers in the hippocampus of PTZ-kindled rats under conditions of rapamycin and axitinib treatment

The aim of the study is to determine level HIF-1α, TNF-α, and NF-kB in hippocampus kindled rats treated with rapamycin axitinib.
 Materials methods. Kindling was produced 29 by administration three-week pentylenetetrazole (PTZ, 35.0 mg/kg, i.p.). Treatment (0.5 i.p.) axitinib (2.5 performed for ten days fully rats. avidin-biotin-peroxidase method used hippocampal slice staining. For negative control, staining using only secondary antibodies.
 Results. HIF-1α expression increased raised 1.77 times compared control (p<0.001). Axitinib treatment resulted 16.7 % (p<0.05) animals, while combined reduced 33.8 (p<0.01). In rats, TNF-α 3.74 greater than Rapamycin 31.0 caused a reduction 21.1 (p<0.05). Combined 48.0 (p<0.001) but still exceeded 1.95 three 19.3 (p>0.05), – 26.5 56.7 
 Conclusions. PTZ-kindling an increase immunoreactivity hippocampus. engendered prevention generalized seizures normalized significant TNF-α. Effects favours synergy action